Smoothed Particle Hydrodynamics for Navier-Stokes Fluid Flow Application

The aim of this publication is to introduce the particle based computational fluid dynamics (CFD) method smoothed particle hydrodynamics (SPH) and introduce an applicable and valid SPH implementation for practical cases. For this purpose, current research approaches are combined regarding performance and numerical stability.  The principles of the method, the mathematical basics and the discretization of the Navier-Stokes equations are clarified. Furthermore, the implementation of method-specific boundary conditions, wall, inlet and outlet, as well as several correction procedures and a surface tension setup into the present code framework are described. The advantages and validity of the method are shown based on different cases. The free surface fluid behavior of a dam break is compared to experimental data of the time dependent water level of selected positions. A Karman vortex street is validated by its Strouhal number for different Reynolds numbers. The frequency of an oscillating drop is analysed and compared to the analytical solution.  The SPH is utilized for pipe flows influenced by a backward facing step and shows an expected qualitative flow field

The use of high-affinity polyhistidine binders as masking probes for the selection of an NDM-1 specific aptamer

The emergence of carbapenemase-producing multi-drug resistant Enterobacteriaceae poses a dramatic, world-wide health risk. Limited treatment options and a lack of easy-to-use methods for the detection of infections with multi-drug resistant bacteria leave the health-care system with a fast-growing challenge. Aptamers are single stranded DNA or RNA molecules that bind to their targets with high affinity and specificity and can therefore serve as outstanding detection probes. However, an effective aptamer selection process is often hampered by non-specific binding. When selections are carried out against recombinant proteins, purification tags (e.g. polyhistidine) serve as attractive side targets, which may impede protein target binding. In this study, aptamer selection was carried out against N-terminally hexa-histidine tagged New Delhi metallo-ß-lactamase 1. After 14 selection rounds binding to polyhistidine was detected rather than to New Delhi metallo-ß-lactamase 1. Hence, the selection strategy was changed. As one aptamer candidate showed remarkable binding affinity to polyhistidine, it was used as a masking probe and selection was restarted from selection round 10. Finally, after three consecutive selection rounds, an aptamer with specific binding properties to New Delhi metallo-ß-lactamase 1 was identified. This aptamer may serve as a much-needed detection probe for New Delhi metallo-ß-lactamase 1 expressing Enterobacteriaceae

Schnellerkennungsmethoden fuer die Eingangskontrolle bei Abfallbeseitigungsanlagen

With 18 refs., 8 tabs., 2 figs.TIB: RN 8908(89-106) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Gutachten zur Verbesserung des Absatzes von Wertstoffen aus Abfaellen

Copy held by FIZ Karlsruhe; available from UB/TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Problemstoffe im Hausmuell sowie im haeuslichen Abwasser bei Kleingewerbebetrieben, 1982 Textbd. Band A: Tabellenbd (Stichprobenergebnisse), Marktstudie Reststoffe im Hausmuell sowie haeuslichen Abwasser ermittelt in ausgewaehlten Branchen. Band B: Tabellenbd zur Marktstudie

TIB: RN 8908 (83-014,2) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman